Hotspot

rps16-trnQ-UUG

LSC · 6056-6852 · 797 bp

Back to Mesembryanthemum

Pi 0.0268

MarkerSeek score 67.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 21
pi: 0.0268
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CCACAACGGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	884-891 bp	1.0	81.5
rps16-trnQ-UUG_p2	CGTCGAGTTAAGAGCATCCC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.2	58.1	931-938 bp	1.0	81.0
rps16-trnQ-UUG_p3	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	858-865 bp	1.0	81.0
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	874-881 bp	1.0	80.9
rps16-trnQ-UUG_p5	CCACAACGGATCATGTCCTT	TAAGGCATCGGGTTTTGGTC	50.0	50.0	57.9	58.2	914-921 bp	1.0	80.7