Hotspot

trnK-UUU-rps16

LSC · 4299-4905 · 607 bp

Back to Mesembryanthemum

Pi 0.015
MarkerSeek score 68.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
607
variable_sites
9
pi
0.015
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA GCTCAACCTACAGAAACCGT 50.0 50.0 58.0 57.8 752-789 bp 1.0 79.8
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC GCTCAACCTACAGAAACCGT 50.0 50.0 58.0 57.8 753-790 bp 1.0 79.8
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GCTCAACCTACAGAAACCGT 50.0 50.0 58.2 57.8 704-741 bp 1.0 78.9
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GCTCAACCTACAGAAACCGT 50.0 50.0 58.2 57.8 703-740 bp 1.0 78.9
trnK-UUU-rps16_p5 CGAATCCCTTGCTTCATCCA AAGAGGGCGGGAATTTTGAA 50.0 45.0 58.0 57.7 719-756 bp 1.0 78.8