Hotspot

psbC

Genome · 27205-28626 · 1422 bp

Back to Meristotropis

Pi 0.00281

MarkerSeek score 45.50

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: Genome
length_bp: 1422
variable_sites: 4
pi: 0.00281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GAGAGATGGCTGAGTGGTTG	TCTTTGCGCCTAGTTTTGGT	55.0	45.0	58.0	58.0	2228-2229 bp	1.0	80.2
psbC_p2	GAGAGATGGCTGAGTGGTTG	TGGTTCTTTGCGCCTAGTTT	55.0	45.0	58.0	58.0	2232-2233 bp	1.0	80.2
psbC_p3	GAGAGATGGCTGAGTGGTTG	TTGGTTCTTTGCGCCTAGTT	55.0	45.0	58.0	58.0	2233-2234 bp	1.0	80.2
psbC_p4	GAGAGATGGCTGAGTGGTTG	GGGCCAATTGCTGTTTTTGT	55.0	45.0	58.0	58.0	2289-2290 bp	1.0	80.1
psbC_p5	GAGAGATGGCTGAGTGGTTG	TGGGCCAATTGCTGTTTTTG	55.0	45.0	58.0	58.0	2290-2291 bp	1.0	80.1