Hotspot

ndhI-ndhG

Genome · 118402-118870 · 469 bp

Back to Meristotropis

Pi 0.0192

MarkerSeek score 52.10

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: Genome
length_bp: 469
variable_sites: 9
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	AATCAAATTGCTTTGGGCCG	CGTTTCAGGCAAATCCAT	45.0	44.4	57.9	53.3	565-587 bp	1.0	41.4
ndhI-ndhG_p2	GCCGTTTACCGATGTCAGTA	CGTTTCAGGCAAATCCAT	50.0	44.4	57.7	53.3	549-571 bp	1.0	41.4
ndhI-ndhG_p3	CAAATTGCTTTGGGCCGTTT	CGTTTCAGGCAAATCCAT	45.0	44.4	58.4	53.3	562-584 bp	1.0	41.4
ndhI-ndhG_p4	AATTGCTTTGGGCCGTTTAC	CGTTTCAGGCAAATCCAT	45.0	44.4	57.6	53.3	560-582 bp	1.0	41.4
ndhI-ndhG_p5	ATCAAATTGCTTTGGGCCGT	CGTTTCAGGCAAATCCAT	45.0	44.4	59.0	53.3	564-586 bp	1.0	41.4