Hotspot

ycf2-trnL-CAA

Genome · 93073-93578 · 506 bp

Back to Meristotropis

Pi 0.0119

MarkerSeek score 63.60

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: Genome
length_bp: 506
variable_sites: 6
pi: 0.0119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	AAAGATGGCTTTTCCCGGAG	AAATGGTAGACACGCGAGAC	50.0	50.0	58.2	58.0	640-704 bp	1.0	79.4
ycf2-trnL-CAA_p2	AAAGATGGCTTTTCCCGGAG	TGAAATGGTAGACACGCGAG	50.0	50.0	58.2	58.0	642-706 bp	1.0	79.4
ycf2-trnL-CAA_p3	AAAGATGGCTTTTCCCGGAG	GCCTTGGTGGTGAAATGGTA	50.0	50.0	58.2	58.1	652-716 bp	1.0	78.8
ycf2-trnL-CAA_p4	AAAGATGGCTTTTCCCGGAG	TGGTGAAATGGTAGACACGC	50.0	50.0	58.2	58.2	645-709 bp	1.0	77.9
ycf2-trnL-CAA_p5	AAAGATGGCTTTTCCCGGAG	GAAATGGTAGACACGCGAGA	50.0	50.0	58.2	57.7	641-705 bp	1.0	77.2