Hotspot

trnW-CCA-trnP-UGG

Genome · 67037-67215 · 179 bp

Back to Meristotropis

Pi 0.0391
MarkerSeek score 75.00
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
Genome
length_bp
179
variable_sites
7
pi
0.0391
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GCGTTCTACCGAACTGAACT GGGTACAAAATGTCACGGGT 50.0 50.0 57.9 58.1 246 bp 1.0 81.0
trnW-CCA-trnP-UGG_p2 GCGTTCTACCGAACTGAACT TGGGTACAAAATGTCACGGG 50.0 50.0 57.9 58.1 247 bp 1.0 81.0
trnW-CCA-trnP-UGG_p3 GCGTTCTACCGAACTGAACT GTCACGGGTTCAAATCCTGT 50.0 50.0 57.9 58.1 235 bp 1.0 81.0
trnW-CCA-trnP-UGG_p4 GCGTTCTACCGAACTGAACT CACGGGTTCAAATCCTGTCA 50.0 50.0 57.9 58.1 233 bp 1.0 81.0
trnW-CCA-trnP-UGG_p5 GCGTTCTACCGAACTGAACT TGTCACGGGTTCAAATCCTG 50.0 50.0 57.9 58.1 236 bp 1.0 81.0