Hotspot

trnR-UCU-trnG-UCC

Genome · 53375-53722 · 348 bp

Back to Meristotropis

Pi 0.0302
MarkerSeek score 68.70
Primer available yes
Label trnR-UCU-trnG-UCC

Feature Metadata

label_name
trnR-UCU-trnG-UCC
feature_type
igs
region
Genome
length_bp
348
variable_sites
10
pi
0.0302
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnG-UCC_p1 TGCGCCCAATAGGATTTGAA AGCCTTCCAAGCTAACGATG 45.0 50.0 57.9 58.0 449-464 bp 1.0 83.4
trnR-UCU-trnG-UCC_p2 TTGCGCCCAATAGGATTTGA AGCCTTCCAAGCTAACGATG 45.0 50.0 57.9 58.0 450-465 bp 1.0 83.4
trnR-UCU-trnG-UCC_p3 TGCGCCCAATAGGATTTGAA CCTAGCCTTCCAAGCTAACG 45.0 55.0 57.9 58.1 452-467 bp 1.0 83.1
trnR-UCU-trnG-UCC_p4 TTGCGCCCAATAGGATTTGA CCTAGCCTTCCAAGCTAACG 45.0 55.0 57.9 58.1 453-468 bp 1.0 83.1
trnR-UCU-trnG-UCC_p5 TGCGCCCAATAGGATTTGAA CCCTAGCCTTCCAAGCTAAC 45.0 55.0 57.9 57.7 453-468 bp 1.0 80.9