Hotspot

atpA-trnR-UCU

Genome · 53048-53302 · 255 bp

Back to Meristotropis

Pi 0.02

MarkerSeek score 58.60

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: Genome
length_bp: 255
variable_sites: 5
pi: 0.02
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	TTTACTGGGGAAGCAGAAGC	TTCAAATCCTATTGGGCGCA	50.0	45.0	57.8	57.9	365-384 bp	1.0	79.4
atpA-trnR-UCU_p2	GGAAGCAGAAGCCCTTTTGA	TTCAAATCCTATTGGGCGCA	50.0	45.0	58.4	57.9	357-376 bp	1.0	78.1
atpA-trnR-UCU_p3	TTTACTGGGGAAGCAGAAGC	AAGAAAAGCGCCCATTGTCT	50.0	45.0	57.8	58.4	424-443 bp	1.0	77.2
atpA-trnR-UCU_p4	TTTACTGGGGAAGCAGAAGC	AAAGAAAAGCGCCCATTGTC	50.0	45.0	57.8	57.6	425-444 bp	1.0	76.7
atpA-trnR-UCU_p5	TTTACTGGGGAAGCAGAAGC	AGAAAAGCGCCCATTGTCTA	50.0	45.0	57.8	57.5	423-442 bp	1.0	76.3