Hotspot

atpI-atpH

Genome · 48451-49470 · 1020 bp

Back to Meristotropis

Pi 0.0134

MarkerSeek score 70.00

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: Genome
length_bp: 1020
variable_sites: 13
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	GCTGCGGCTTATATAGGTGA	CGCAATCCCTTCTACAGCTT	50.0	50.0	57.3	58.0	1141-1182 bp	1.0	78.1
atpI-atpH_p2	GCTGCGGCTTATATAGGTGA	AACAGAAGCGGCAGAAATCA	50.0	45.0	57.3	58.1	1051-1092 bp	1.0	77.8
atpI-atpH_p3	ACAAGCGGTATTCAAGCTCT	CGCAATCCCTTCTACAGCTT	45.0	50.0	57.2	58.0	1177-1218 bp	1.0	77.7
atpI-atpH_p4	GGTGAATCCATGGAAGGTCA	CGCAATCCCTTCTACAGCTT	50.0	50.0	57.2	58.0	1126-1167 bp	1.0	77.6
atpI-atpH_p5	GCTGCGGCTTATATAGGTGA	GTCCAATAGAAGCAAGCCCA	50.0	50.0	57.3	57.9	1091-1132 bp	1.0	77.5