Hotspot

petN-trnC-GCA

Genome · 33803-34870 · 1068 bp

Back to Meristotropis

Pi 0.0169

MarkerSeek score 60.70

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: Genome
length_bp: 1068
variable_sites: 18
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	45.0	50.0	58.0	58.1	1145-1179 bp	1.0	80.9
petN-trnC-GCA_p2	CAGCCCAAGCAAGACTTACT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.8	58.1	1139-1173 bp	1.0	79.4
petN-trnC-GCA_p3	TTAAAGCAGCCCAAGCAAGA	GGGGGACTGCAAATCCTTTT	45.0	50.0	58.0	58.4	1163-1197 bp	1.0	78.7
petN-trnC-GCA_p4	AGAGTCCACTTCTTCCCCAT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.6	58.1	1199-1233 bp	1.0	78.0
petN-trnC-GCA_p5	CAGCCCAAGCAAGACTTACT	GGGGGACTGCAAATCCTTTT	50.0	50.0	57.8	58.4	1157-1191 bp	1.0	77.2