Hotspot

trnS-UGA-psbC

Genome · 26994-27204 · 211 bp

Back to Meristotropis

Pi 0.0476

MarkerSeek score 65.40

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: Genome
length_bp: 211
variable_sites: 10
pi: 0.0476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GAGAGATGGCTGAGTGGTTG	CGTCGTTCCGCGGAATATAT	55.0	50.0	58.0	58.0	557-558 bp	1.0	81.3
trnS-UGA-psbC_p2	GAGAGATGGCTGAGTGGTTG	TCTAAGGGGTCCCAATGGTT	55.0	50.0	58.0	57.9	621-622 bp	1.0	81.0
trnS-UGA-psbC_p3	CCCACCCCTTCCTTTAATCG	CGTCGTTCCGCGGAATATAT	55.0	50.0	57.9	58.0	850-851 bp	1.0	80.5
trnS-UGA-psbC_p4	CTTCACAAAAGGGCATGCTG	CGTCGTTCCGCGGAATATAT	50.0	50.0	57.9	58.0	719-720 bp	1.0	80.5
trnS-UGA-psbC_p5	GAGAGATGGCTGAGTGGTTG	CACTGGACCAGAAGCTTCTC	55.0	55.0	58.0	57.9	813-814 bp	1.0	80.4