Pi
0.00708
MarkerSeek score
67.00
Primer available
yes
Label
trnL-UAA-trnT-UGU
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnL-UAA-trnT-UGU_p1 | ACCAATTTCGCCATATCCCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.0 | 57.9 | 1616-1679 bp | 1.0 | 83.1 |
| trnL-UAA-trnT-UGU_p2 | ACCAATTTCGCCATATCCCC | GAGTCGAACCGATGACCATC | 50.0 | 55.0 | 58.0 | 58.2 | 1647-1710 bp | 1.0 | 82.8 |
| trnL-UAA-trnT-UGU_p3 | TAGCGTCTACCAATTTCGCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.1 | 57.9 | 1624-1687 bp | 1.0 | 82.6 |
| trnL-UAA-trnT-UGU_p4 | TAGCGTCTACCAATTTCGCC | GAGTCGAACCGATGACCATC | 50.0 | 55.0 | 58.1 | 58.2 | 1655-1718 bp | 1.0 | 82.3 |
| trnL-UAA-trnT-UGU_p5 | AGTCCGTAGCGTCTACCAAT | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.2 | 57.9 | 1630-1693 bp | 1.0 | 81.2 |