Hotspot

trnL-UAG-ccsA

SSC · 118949-119049 · 101 bp

Back to Mentzelia

Pi 0.07
MarkerSeek score 75.80
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
101
variable_sites
7
pi
0.07
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA ACAATTGAAACAATCGAAAAGGAGA 50.0 32.0 57.9 58.0 223-224 bp 1.0 42.9
trnL-UAG-ccsA_p2 GTAGACACGCTGCTCTTAGG ACAATTGAAACAATCGAAAAGGAGA 55.0 32.0 57.8 58.0 224-225 bp 1.0 42.9
trnL-UAG-ccsA_p3 TGAAATCGGTAGACACGCTG ACAATTGAAACAATCGAAAAGGAGA 50.0 32.0 58.0 58.0 232-233 bp 1.0 42.8
trnL-UAG-ccsA_p4 GGTAGACACGCTGCTCTTAG ACAATTGAAACAATCGAAAAGGAGA 55.0 32.0 57.8 58.0 225-226 bp 1.0 42.8
trnL-UAG-ccsA_p5 GTGAAATCGGTAGACACGCT ACAATTGAAACAATCGAAAAGGAGA 50.0 32.0 58.0 58.0 233-234 bp 1.0 42.7