Hotspot

rpl32-trnL-UAG

SSC · 117896-118868 · 973 bp

Back to Mentzelia

Pi 0.0526

MarkerSeek score 81.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 973
variable_sites: 50
pi: 0.0526
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGAAGGGATATTGGGCAGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.6	58.0	1101-1105 bp	1.0	82.3
rpl32-trnL-UAG_p2	AAGAAGGGATATTGGGCAGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.6	58.0	1102-1106 bp	1.0	82.2
rpl32-trnL-UAG_p3	ATATTGGGCAGCGCTAAAGG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.4	58.0	1093-1097 bp	1.0	82.1
rpl32-trnL-UAG_p4	ATATTGGGCAGCGCTAAAGG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.4	58.0	1094-1098 bp	1.0	82.1
rpl32-trnL-UAG_p5	GGATATTGGGCAGCGCTAAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.4	58.0	1095-1099 bp	1.0	82.1