Hotspot

ndhF-rpl32

SSC · 116566-117733 · 1168 bp

Back to Mentzelia

Pi 0.0578

MarkerSeek score 84.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1168
variable_sites: 65
pi: 0.0578
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	GCTGCCCAATATCCCTTCTT	50.0	50.0	58.0	57.6	1349-1351 bp	1.0	83.2
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	GCTGCCCAATATCCCTTCTT	45.0	50.0	58.0	57.6	1347-1349 bp	1.0	83.1
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	GCTGCCCAATATCCCTTCTT	45.0	50.0	58.0	57.6	1348-1350 bp	1.0	83.1
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	CCTTTAGCGCTGCCCAATAT	50.0	50.0	58.0	58.4	1357-1359 bp	1.0	83.0
ndhF-rpl32_p5	GTTGCCGTCGGAAAAAGAAG	TTTAGCGCTGCCCAATATCC	50.0	50.0	58.0	58.4	1355-1357 bp	1.0	82.9