Hotspot

trnW-CCA-trnP-UGG

LSC · 70308-70485 · 178 bp

Back to Mentzelia

Pi 0.107
MarkerSeek score 79.80
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
178
variable_sites
19
pi
0.107
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GTTTTGGAGACCCACGTTCT GTCACGGGTTCAAATCCTGT 50.0 50.0 58.0 58.1 247-263 bp 1.0 84.8
trnW-CCA-trnP-UGG_p2 GTTTTGGAGACCCACGTTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 258-274 bp 1.0 84.6
trnW-CCA-trnP-UGG_p3 GTTTTGGAGACCCACGTTCT TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 259-275 bp 1.0 84.6
trnW-CCA-trnP-UGG_p4 GTTTTGGAGACCCACGTTCT ATTGAAGGGATGTAGCGCAG 50.0 50.0 58.0 58.0 298-314 bp 1.0 84.3
trnW-CCA-trnP-UGG_p5 ACCTACGACATCGGGTTTTG ATTGAAGGGATGTAGCGCAG 50.0 50.0 57.9 58.0 312-328 bp 1.0 83.7