Hotspot

ndhC-trnV-UAC

LSC · 53371-54559 · 1189 bp

Back to Mentzelia

Pi 0.0474
MarkerSeek score 78.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1189
variable_sites
51
pi
0.0474
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTCCGGAAATGAAAAATGCCA AGAAGGTCTACGGTTCGAGT 42.9 50.0 57.4 57.8 1230-1310 bp 1.0 72.9
ndhC-trnV-UAC_p2 ACTCCGGAAATGAAAAATGCC AGAAGGTCTACGGTTCGAGT 42.9 50.0 57.4 57.8 1231-1311 bp 1.0 72.9
ndhC-trnV-UAC_p3 TCCGGAAATGAAAAATGCCA AGAAGGTCTACGGTTCGAGT 40.0 50.0 56.2 57.8 1229-1309 bp 1.0 71.2
ndhC-trnV-UAC_p4 CTCCGGAAATGAAAAATGCCA GAAGGTCTACGGTTCGAGTC 42.9 55.0 57.4 57.5 1229-1309 bp 1.0 70.2
ndhC-trnV-UAC_p5 ACTCCGGAAATGAAAAATGCC GAAGGTCTACGGTTCGAGTC 42.9 55.0 57.4 57.5 1230-1310 bp 1.0 70.1