Hotspot

rps16-trnQ-UUG

LSC · 6323-7986 · 1664 bp

Back to Mentzelia

Pi 0.0457
MarkerSeek score 76.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1664
variable_sites
74
pi
0.0457
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1737-1757 bp 1.0 83.7
rps16-trnQ-UUG_p2 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1751-1771 bp 1.0 81.8
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1741-1761 bp 1.0 78.9
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 1767-1787 bp 1.0 78.8
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TAGAAATCGAAATGGGGCGT 45.0 45.0 58.1 57.3 1797-1817 bp 1.0 78.5