Hotspot

trnK-UUU-rps16

LSC · 4292-5161 · 870 bp

Back to Mentzelia

Pi 0.0506
MarkerSeek score 75.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
870
variable_sites
44
pi
0.0506
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT ACTTCGGTCTAATCAAATAAAAATCA 50.0 26.9 58.2 55.8 935-962 bp 1.0 43.8
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT ACTTCGGTCTAATCAAATAAAAATCA 50.0 26.9 58.2 55.8 934-961 bp 1.0 43.8
trnK-UUU-rps16_p3 CCGATGGTATGGACGAATCC ACTTCGGTCTAATCAAATAAAAATCA 55.0 26.9 57.9 55.8 996-1023 bp 1.0 43.6
trnK-UUU-rps16_p4 CGATGGTATGGACGAATCCC ACTTCGGTCTAATCAAATAAAAATCA 55.0 26.9 57.9 55.8 995-1022 bp 1.0 43.6
trnK-UUU-rps16_p5 TGGTATGGACGAATCCCTCA ACTTCGGTCTAATCAAATAAAAATCA 50.0 26.9 57.8 55.8 992-1019 bp 1.0 43.6