Hotspot

ycf1

SSC · 122054-127612 · 5559 bp

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Pi 0.00551
MarkerSeek score 41.80
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5559
variable_sites
106
pi
0.00551
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCTCTAACAAATTTACTGATGAAA TCCTGAACGGATCATATCGGA 25.0 47.6 52.4 58.1 232 bp 1.0 42.4
ycf1_p2 TTCTCTAACAAATTTACTGATGAA TCCTGAACGGATCATATCGGA 25.0 47.6 52.4 58.1 233 bp 1.0 42.4
ycf1_p3 TCTCTAACAAATTTACTGATGAAA CCTGAACGGATCATATCGGA 25.0 50.0 52.4 56.6 231 bp 1.0 42.4
ycf1_p4 TCTCTAACAAATTTACTGATGAAA TCCTGAACGGATCATATCGG 25.0 50.0 52.4 56.6 232 bp 1.0 42.4
ycf1_p5 TTCTCTAACAAATTTACTGATGAA CCTGAACGGATCATATCGGA 25.0 50.0 52.4 56.6 232 bp 1.0 42.4