Hotspot

rps3

LSC · 81860-82522 · 663 bp

Back to Mentha

Pi 0.00375
MarkerSeek score 50.90
Primer available yes
Label rps3

Feature Metadata

label_name
rps3
feature_type
gene
region
LSC
length_bp
663
variable_sites
9
pi
0.00375
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3_p1 TGAACGAGTTTTCCCTTTTTCC AACTGTGGTAGATCGCGATG 40.9 50.0 57.4 57.8 766 bp 1.0 58.1
rps3_p2 TGAACGAGTTTTCCCTTTTTCC CAACTGTGGTAGATCGCGAT 40.9 50.0 57.4 57.8 767 bp 1.0 58.1
rps3_p3 ACGAGTTTTCCCTTTTTCCTTC AACTGTGGTAGATCGCGATG 40.9 50.0 57.1 57.8 763 bp 1.0 56.0
rps3_p4 ACGAGTTTTCCCTTTTTCCTTC CAACTGTGGTAGATCGCGAT 40.9 50.0 57.1 57.8 764 bp 1.0 56.0
rps3_p5 GAACGAGTTTTCCCTTTTTCCT AACTGTGGTAGATCGCGATG 40.9 50.0 57.1 57.8 765 bp 1.0 56.0