Hotspot

ndhD

SSC · 113850-115379 · 1530 bp

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Pi 0.00267
MarkerSeek score 35.50
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1530
variable_sites
13
pi
0.00267
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 TTGGAGTTGGGACCCAAAAG ATGTACCCAATGTGTCCGTG 50.0 50.0 57.9 57.9 2274-2280 bp 1.0 80.0
ndhD_p2 TTGGAGTTGGGACCCAAAAG ACTGCGTTGGTTGTAAGAGG 50.0 50.0 57.9 58.1 2173-2179 bp 1.0 79.9
ndhD_p3 TTGGAGTTGGGACCCAAAAG CTGCGTTGGTTGTAAGAGGT 50.0 50.0 57.9 58.1 2172-2178 bp 1.0 79.9
ndhD_p4 TTGGAGTTGGGACCCAAAAG GTGCCCCACCGATGTATTAG 50.0 55.0 57.9 58.1 2253-2259 bp 1.0 79.9
ndhD_p5 CTAACGAAGCATGGGGATCC ATGTACCCAATGTGTCCGTG 55.0 50.0 58.1 57.9 2296-2302 bp 1.0 79.7