Hotspot

accD

LSC · 57069-58550 · 1482 bp

Pi 0.00187

MarkerSeek score 30.80

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1482
variable_sites: 11
pi: 0.00187
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AGACAGCGGAAAAACCCTTT	AATGAGATTGGGTCCCCTCA	45.0	50.0	57.9	58.0	2355-2373 bp	1.0	81.2
accD_p2	AGACAGCGGAAAAACCCTTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	2291-2309 bp	1.0	81.1
accD_p3	TGTTCGATGCGAAGAGACAG	AATGAGATTGGGTCCCCTCA	50.0	50.0	58.0	58.0	2369-2387 bp	0.8	73.5
accD_p4	TGTTCGATGCGAAGAGACAG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	2305-2323 bp	0.8	73.5
accD_p5	TGTTCGATGCGAAGAGACAG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	2311-2329 bp	0.8	72.9