Hotspot

trnT-UGU-trnL-UAA

LSC · 46276-46942 · 667 bp

Back to Mentha

Pi 0.00828
MarkerSeek score 62.10
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
667
variable_sites
15
pi
0.00828
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 701-740 bp 1.0 81.7
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 733-772 bp 1.0 80.7
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 695-734 bp 1.0 80.5
trnT-UGU-trnL-UAA_p4 GGAATCGAACCGATGACCAT CGTCTACCGATTTCGCCATA 50.0 50.0 57.8 57.6 727-766 bp 1.0 79.4
trnT-UGU-trnL-UAA_p5 CTAACCTCTGAGCTAAGCGG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.5 58.2 693-732 bp 1.0 78.9