Hotspot

rps4-trnT-UGU

LSC · 45764-46202 · 439 bp

Back to Mentha

Pi 0.0081
MarkerSeek score 59.00
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
439
variable_sites
11
pi
0.0081
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.95
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 TCTAAGATCACTCCCGGCTT CTCAGAGGTTAGAGCATCGC 50.0 55.0 57.8 57.9 572-614 bp 1.0 83.2
rps4-trnT-UGU_p2 TTCTAAGATCACTCCCGGCT CTCAGAGGTTAGAGCATCGC 50.0 55.0 57.8 57.9 573-615 bp 1.0 83.2
rps4-trnT-UGU_p3 TCTAAGATCACTCCCGGCTT ATGGTCATCGGTTCGATTCC 50.0 50.0 57.8 57.8 540-582 bp 1.0 82.4
rps4-trnT-UGU_p4 TTCTAAGATCACTCCCGGCT ATGGTCATCGGTTCGATTCC 50.0 50.0 57.8 57.8 541-583 bp 1.0 82.4
rps4-trnT-UGU_p5 GTTAGTCCTGGTAAAGCCCC CTCAGAGGTTAGAGCATCGC 55.0 55.0 57.6 57.9 538-580 bp 1.0 81.2