Hotspot

ycf3-trnS-GGA

LSC · 43914-44783 · 870 bp

Back to Mentha

Pi 0.0061
MarkerSeek score 55.90
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
870
variable_sites
15
pi
0.0061
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TGCCTCCTTTTCTCCTGAAG CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 1011-1042 bp 1.0 76.1
ycf3-trnS-GGA_p2 GCCTCCTTTTCTCCTGAAGT CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 1010-1041 bp 1.0 76.1
ycf3-trnS-GGA_p3 TGCCTCCTTTTCTCCTGAAG AAGTCAAGGTGCGGAAAGAG 50.0 50.0 57.1 58.1 1042-1073 bp 1.0 75.8
ycf3-trnS-GGA_p4 GCCTCCTTTTCTCCTGAAGT AAGTCAAGGTGCGGAAAGAG 50.0 50.0 57.1 58.1 1041-1072 bp 1.0 75.8
ycf3-trnS-GGA_p5 TGCCTCCTTTTCTCCTGAAG TTCCAATGCTACGCCTTGAA 50.0 45.0 57.1 57.8 982-1013 bp 1.0 75.4