Hotspot

psaA-ycf3

LSC · 41240-41967 · 728 bp

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Pi 0.00479
MarkerSeek score 55.50
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
728
variable_sites
13
pi
0.00479
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCCCATTCCTCGAAAGAAGT ATTGGTTGAAGATCACGGGG 50.0 50.0 57.9 57.9 840-882 bp 1.0 82.4
psaA-ycf3_p2 GAAAAATGACCCGGTTTGGC ATTGGTTGAAGATCACGGGG 50.0 50.0 58.2 57.9 858-900 bp 1.0 81.5
psaA-ycf3_p3 GCCCATTCCTCGAAAGAAGT TGGTTTGATCAAGCTGCTGA 50.0 45.0 57.9 57.7 913-955 bp 1.0 80.9
psaA-ycf3_p4 GAAAAATGACCCGGTTTGGC TGGTTTGATCAAGCTGCTGA 50.0 45.0 58.2 57.7 931-973 bp 1.0 80.0
psaA-ycf3_p5 GCCCATTCCTCGAAAGAAGT CGGAGGCTTGGTTTGATCAA 50.0 50.0 57.9 58.5 921-963 bp 1.0 79.3