Hotspot

trnE-UUC-trnT-GGU

LSC · 30316-30869 · 554 bp

Back to Mentha

Pi 0.00686
MarkerSeek score 52.40
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
554
variable_sites
14
pi
0.00686
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 695-718 bp 1.0 81.0
trnE-UUC-trnT-GGU_p2 TTTCGTAGTACCCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 674-697 bp 1.0 80.7
trnE-UUC-trnT-GGU_p3 TTTCGTAGTACCCTACCCCC TGGCGTTACTCTACCACTGA 55.0 50.0 57.8 58.1 671-694 bp 1.0 80.5
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 657-680 bp 1.0 80.3
trnE-UUC-trnT-GGU_p5 TTTCGTAGTACCCTACCCCC CCGATGACTTACGCCTTACC 55.0 55.0 57.8 58.2 692-715 bp 1.0 79.9