Hotspot

atpH-atpI

LSC · 13560-14552 · 993 bp

Back to Mentha

Pi 0.00425
MarkerSeek score 55.90
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
993
variable_sites
14
pi
0.00425
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AACGGAAGCAGCAGAAATCA TCCCTGTCATGTTCCTTGGA 45.0 50.0 58.1 58.3 1126-1154 bp 1.0 81.0
atpH-atpI_p2 AGCCAATCCAGCAGCAATAA TCCCTGTCATGTTCCTTGGA 45.0 50.0 57.9 58.3 1144-1172 bp 1.0 80.6
atpH-atpI_p3 GCCAATCCAGCAGCAATAAC TCCCTGTCATGTTCCTTGGA 50.0 50.0 57.8 58.3 1143-1171 bp 1.0 80.1
atpH-atpI_p4 TCCAGCAGCAATAACGGAAG TCCCTGTCATGTTCCTTGGA 50.0 50.0 58.3 58.3 1138-1166 bp 1.0 79.7
atpH-atpI_p5 AACGGAAGCAGCAGAAATCA TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.1 58.5 1078-1106 bp 1.0 79.1