Hotspot

rps16-trnQ-UUG

LSC · 5961-6955 · 995 bp

Back to Mentha

Pi 0.0121
MarkerSeek score 63.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
995
variable_sites
32
pi
0.0121
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.96
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAAATTGAAATGGGGCGTGG 45.0 50.0 58.1 58.0 1093-1152 bp 1.0 84.8
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1035-1094 bp 1.0 84.6
rps16-trnQ-UUG_p3 GCCAAGAGCACCTCATTTCT GAAATTGAAATGGGGCGTGG 50.0 50.0 58.2 58.0 1164-1223 bp 1.0 84.3
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GTCTCGCTATTCGGAGGTTC 45.0 55.0 58.1 57.9 1048-1107 bp 1.0 84.2
rps16-trnQ-UUG_p5 GCCAAGAGCACCTCATTTCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1106-1165 bp 1.0 84.1