Hotspot

ndhH

SSC · 121439-122614 · 1176 bp

Back to Menonvillea

Pi 0.0255

MarkerSeek score 59.00

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1176
variable_sites: 30
pi: 0.0255
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	ACTAATCGATTGGGCAGCAG	GAAAACGTCAACGACTGCTG	50.0	50.0	58.0	58.0	1895-1896 bp	1.0	81.7
ndhH_p2	CTGTCGGCTAGAGCTTGAAG	GAAAACGTCAACGACTGCTG	55.0	50.0	58.1	58.0	1616-1622 bp	1.0	81.3
ndhH_p3	CAACGAATCGCACGTAGAGA	GAAAACGTCAACGACTGCTG	50.0	50.0	58.1	58.0	1951-1952 bp	1.0	81.1
ndhH_p4	TACGTTGTTGTATCCCTGCG	GAAAACGTCAACGACTGCTG	50.0	50.0	58.0	58.0	1564 bp	0.5	60.0
ndhH_p5	TACGTTGTTGTATCCCTGCG	AAAAAGAAGCAAGCAGGGGA	50.0	45.0	58.0	57.9	1696 bp	0.5	59.3