Hotspot

ndhG-ndhI

SSC · 118391-118725 · 335 bp

Back to Menonvillea

Pi 0.0129

MarkerSeek score 49.80

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 335
variable_sites: 4
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GTGCAGCAGCTACAAAATGG	TCAACAAACCCTACGAGCTG	50.0	50.0	58.0	57.8	992-1012 bp	1.0	83.9
ndhG-ndhI_p2	GTGCAGCAGCTACAAAATGG	GTCAACAAACCCTACGAGCT	50.0	50.0	58.0	57.8	993-1013 bp	1.0	83.9
ndhG-ndhI_p3	ACCTCCCACTATAAGACCGG	TCAACAAACCCTACGAGCTG	55.0	50.0	57.9	57.8	877-897 bp	1.0	83.9
ndhG-ndhI_p4	GCCCCCACGTAAATAAGGAG	TCAACAAACCCTACGAGCTG	55.0	50.0	58.0	57.8	1014-1034 bp	1.0	83.6
ndhG-ndhI_p5	GCCCCCACGTAAATAAGGAG	GTCAACAAACCCTACGAGCT	55.0	50.0	58.0	57.8	1015-1035 bp	1.0	83.6