Hotspot

rpl32-trnL

SSC · 113002-113733 · 732 bp

Back to Menonvillea

Pi 0.0431

MarkerSeek score 77.90

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 732
variable_sites: 30
pi: 0.0431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	AGGGAAATCACTTTCGACAGG	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.9	57.9	798-829 bp	1.0	77.0
rpl32-trnL_p2	AGGGAAATCACTTTCGACAGG	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.9	57.8	791-822 bp	1.0	76.6
rpl32-trnL_p3	AGGGAAATCACTTTCGACAGG	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.9	57.8	797-828 bp	1.0	76.5
rpl32-trnL_p4	AGGGAAATCACTTTCGACAGG	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.9	57.8	796-827 bp	1.0	76.5
rpl32-trnL_p5	AGGGAAATCACTTTCGACAGG	GAGCAGCGTGTCTACCAATT	47.6	50.0	57.9	58.3	792-823 bp	1.0	75.9