Hotspot

ndhF-rpl32

SSC · 111984-112842 · 859 bp

Back to Menonvillea

Pi 0.038
MarkerSeek score 69.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
859
variable_sites
32
pi
0.038
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TCCATGAATATTGATATGTATGTTCCA GCTTTTTCGAGATAGAAGTACGT 29.6 39.1 56.7 56.7 927-930 bp 1.0 44.3
ndhF-rpl32_p2 CCATGAATATTGATATGTATGTTCCA GCTTTTTCGAGATAGAAGTACGT 30.8 39.1 55.5 56.7 926-929 bp 1.0 44.3
ndhF-rpl32_p3 TCCATGAATATTGATATGTATGTTCCA GCTTTTTCGAGATAGAAGTACGTT 29.6 37.5 56.7 57.4 927-930 bp 1.0 44.3
ndhF-rpl32_p4 TCCATGAATATTGATATGTATGTTCC GCTTTTTCGAGATAGAAGTACGT 30.8 39.1 55.3 56.7 927-930 bp 1.0 44.3
ndhF-rpl32_p5 CCATGAATATTGATATGTATGTTCCA GCTTTTTCGAGATAGAAGTACGTT 30.8 37.5 55.5 57.4 926-929 bp 1.0 44.3