Hotspot

rpl36-rps8

LSC · 78695-79164 · 470 bp

Back to Menonvillea

Pi 0.0384

MarkerSeek score 68.90

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 470
variable_sites: 18
pi: 0.0384
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	CGGACGGAAGCCCTTATTTT	TCGGCGGAGAAATTTTGTGT	50.0	45.0	58.3	58.4	525-534 bp	1.0	77.6
rpl36-rps8_p2	CGGACGGAAGCCCTTATTTT	CGGCGGAGAAATTTTGTGTT	50.0	45.0	58.3	57.6	524-533 bp	1.0	77.5
rpl36-rps8_p3	CCCTCCTACGGATTAGTCGA	TCGGCGGAGAAATTTTGTGT	55.0	45.0	57.7	58.4	563-572 bp	1.0	77.3
rpl36-rps8_p4	CCCTCCTACGGATTAGTCGA	CGGCGGAGAAATTTTGTGTT	55.0	45.0	57.7	57.6	562-571 bp	1.0	77.2
rpl36-rps8_p5	TAATTCGTCCCCTCCTACGG	TCGGCGGAGAAATTTTGTGT	55.0	45.0	58.3	58.4	572-581 bp	1.0	77.0