Hotspot

psbL-psbF

LSC · 63419-63439 · 21 bp

Back to Menonvillea

Pi 0.0476

MarkerSeek score 75.30

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 21
variable_sites: 1
pi: 0.0476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	AACCCCCAATAGAGACTGGT	TGGACTAGCTGTACCTACCG	50.0	55.0	57.6	58.0	153 bp	1.0	77.2
psbL-psbF_p2	AACCCCCAATAGAGACTGGT	GGACTAGCTGTACCTACCGT	50.0	55.0	57.6	58.0	152 bp	1.0	77.2
psbL-psbF_p3	AACCCCCAATAGAGACTGGT	TTGGCTGTTCATGGACTAGC	50.0	50.0	57.6	57.9	164 bp	1.0	76.7
psbL-psbF_p4	AACCCCCAATAGAGACTGGT	TCAGCAATGCAGTTCATCCA	50.0	45.0	57.6	57.8	113 bp	1.0	76.2
psbL-psbF_p5	AACCCCCAATAGAGACTGGT	GGCTGTTCATGGACTAGCTG	50.0	55.0	57.6	58.3	162 bp	1.0	74.9