Hotspot

trnS-ycf9

LSC · 34983-35329 · 347 bp

Back to Menonvillea

Pi 0.0289

MarkerSeek score 56.40

Primer available yes

Label trnS-ycf9

Feature Metadata

label_name: trnS-ycf9
feature_type: igs
region: LSC
length_bp: 347
variable_sites: 10
pi: 0.0289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-ycf9_p1	TGTCTCTCCGAGAAGTTGGT	TGGGAGGGGTCATCTCATTT	50.0	50.0	58.0	58.0	1014-1019 bp	1.0	82.2
trnS-ycf9_p2	TTAATGCGTTCTCCGACTGG	TGGGAGGGGTCATCTCATTT	50.0	50.0	58.0	58.0	1250-1255 bp	1.0	82.0
trnS-ycf9_p3	TGTCTCTCCGAGAAGTTGGT	CACTCTTTGGTTTGGGGTCA	50.0	50.0	58.0	57.9	1421-1422 bp	1.0	81.7
trnS-ycf9_p4	TGTCTCTCCGAGAAGTTGGT	ACACTCTTTGGTTTGGGGTC	50.0	50.0	58.0	57.9	1422-1423 bp	1.0	81.7
trnS-ycf9_p5	TTAATGCGTTCTCCGACTGG	CACTCTTTGGTTTGGGGTCA	50.0	50.0	58.0	57.9	1657-1658 bp	1.0	81.5