Hotspot

ycf6-psbM

LSC · 27863-28414 · 552 bp

Back to Menonvillea

Pi 0.0275

MarkerSeek score 74.60

Primer available yes

Label ycf6-psbM

Feature Metadata

label_name: ycf6-psbM
feature_type: igs
region: LSC
length_bp: 552
variable_sites: 15
pi: 0.0275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf6-psbM_p1	TCGTAGTGTGGGGAAGAAGT	TGCTACTGCACTCTTCATTCT	50.0	42.9	58.0	57.0	657-660 bp	1.0	66.8
ycf6-psbM_p2	CGTAGTGTGGGGAAGAAGTG	TGCTACTGCACTCTTCATTCT	55.0	42.9	57.9	57.0	656-659 bp	1.0	66.2
ycf6-psbM_p3	CTCTCGTAGTGTGGGGAAGA	TGCTACTGCACTCTTCATTCT	55.0	42.9	58.2	57.0	660-663 bp	1.0	65.5
ycf6-psbM_p4	CTCTCTCGTAGTGTGGGGAA	TGCTACTGCACTCTTCATTCT	55.0	42.9	58.2	57.0	662-665 bp	1.0	65.5
ycf6-psbM_p5	TCTCTCGTAGTGTGGGGAAG	TGCTACTGCACTCTTCATTCT	55.0	42.9	58.2	57.0	661-664 bp	1.0	65.5