Hotspot

trnK-rps16

LSC · 4276-5013 · 738 bp

Back to Menonvillea

Pi 0.0323

MarkerSeek score 73.50

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 738
variable_sites: 22
pi: 0.0323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	AAAGCCGAGTACTCTACCGT	CGCTCAACCAACAAGAACAG	50.0	50.0	58.2	57.6	810-860 bp	1.0	81.6
trnK-rps16_p2	AAGCCGAGTACTCTACCGTT	CGCTCAACCAACAAGAACAG	50.0	50.0	58.2	57.6	809-859 bp	1.0	81.6
trnK-rps16_p3	CAAACTCTACCGCGGATCTG	CGCTCAACCAACAAGAACAG	55.0	50.0	58.4	57.6	880-930 bp	1.0	79.3
trnK-rps16_p4	TACCGTTGAGTTAGCAACCC	CGCTCAACCAACAAGAACAG	50.0	50.0	57.5	57.6	796-846 bp	1.0	79.3
trnK-rps16_p5	AGTCGCACTTAAAAGCCGAG	CGCTCAACCAACAAGAACAG	50.0	50.0	58.6	57.6	821-871 bp	1.0	78.4