Hotspot

trnH-psbA

LSC · 78-328 · 251 bp

Back to Menonvillea

Pi 0.084

MarkerSeek score 78.10

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 251
variable_sites: 20
pi: 0.084
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	309-372 bp	1.0	86.2
trnH-psbA_p2	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	50.0	55.0	58.2	58.1	310-373 bp	1.0	86.0
trnH-psbA_p3	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	324-387 bp	1.0	85.8
trnH-psbA_p4	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	325-388 bp	1.0	85.6
trnH-psbA_p5	AATCCACTTGGCTACATCCG	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.7	58.1	302-365 bp	1.0	84.8