Hotspot

ndhF-rpl32

SSC · 112633-113489 · 857 bp

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Pi 0.0459
MarkerSeek score 76.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
857
variable_sites
58
pi
0.0459
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AAAAGTCCACATACGGCGAA GGAGTTGGAACTTCAACCCA 45.0 50.0 57.8 57.6 1690 bp 0.333333 49.1
ndhF-rpl32_p2 GCAATAGGTCGTGTGAACCA GGAGTTGGAACTTCAACCCA 50.0 50.0 58.2 57.6 2076 bp 0.333333 48.9
ndhF-rpl32_p3 TGCAATAGGTCGTGTGAACC GGAGTTGGAACTTCAACCCA 50.0 50.0 58.2 57.6 2077 bp 0.333333 48.9
ndhF-rpl32_p4 GGTCGTGTGAACCAAAAACC GGAGTTGGAACTTCAACCCA 50.0 50.0 57.8 57.6 2070 bp 0.333333 48.9
ndhF-rpl32_p5 AAAGTCCACATACGGCGAAG GGAGTTGGAACTTCAACCCA 50.0 50.0 58.3 57.6 1689 bp 0.333333 48.2