Hotspot

ndhJ-psbG

LSC · 49036-49137 · 102 bp

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Pi 0.0523
MarkerSeek score 72.00
Primer available yes
Label ndhJ-psbG

Feature Metadata

label_name
ndhJ-psbG
feature_type
igs
region
LSC
length_bp
102
variable_sites
8
pi
0.0523
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ-psbG_p1 CCTTGGTAATCGAAGCCCAA TCTTCCCACGAATTAGTGAA 50.0 40.0 57.9 54.0 200 bp 1.0 50.3
ndhJ-psbG_p2 CCTTGGTAATCGAAGCCCAA CAGTATCTTCCCACGAATTAGTGA 50.0 41.7 57.9 58.1 205 bp 1.0 49.4
ndhJ-psbG_p3 GCCCAACGATCTATGAACCA TCTTCCCACGAATTAGTGAA 50.0 40.0 57.7 54.0 186 bp 1.0 48.9
ndhJ-psbG_p4 CCTTGGTAATCGAAGCCCAA AGTATCTTCCCACGAATTAGTGA 50.0 39.1 57.9 56.8 204 bp 1.0 48.6
ndhJ-psbG_p5 CCTTGGTAATCGAAGCCCAA CAGTATCTTCCCACGAATTAGTG 50.0 43.5 57.9 56.8 205 bp 1.0 48.3