Hotspot

trnL-trnF

LSC · 47081-47902 · 822 bp

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Pi 0.0949
MarkerSeek score 84.80
Primer available yes
Label trnL-trnF

Feature Metadata

label_name
trnL-trnF
feature_type
igs
region
LSC
length_bp
822
variable_sites
86
pi
0.0949
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-trnF_p1 AATCGTGAGGGTTCAAGTCC TACCAACTGAGCTATCCCGG 50.0 55.0 57.5 58.6 712-874 bp 1.0 83.5
trnL-trnF_p2 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 750-912 bp 0.666667 71.8
trnL-trnF_p3 AATCGTGAGGGTTCAAGTCC GAACTGGTGACACGAGGATT 50.0 50.0 57.5 57.5 747-909 bp 0.666667 68.5
trnL-trnF_p4 TTCAAGTCCCTCTATCCCCA TTTGAACTGGTGACACGAGG 50.0 50.0 57.0 58.1 739-901 bp 0.666667 67.9
trnL-trnF_p5 AATCGTGAGGGTTCAAGTCC TGAACTGGTGACACGAGGAT 50.0 50.0 57.5 58.7 748-910 bp 0.666667 66.9