Hotspot

atpH-atpI

LSC · 13381-13880 · 500 bp

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Pi 0.0327
MarkerSeek score 70.90
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
500
variable_sites
24
pi
0.0327
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 TACCTTGACCAACTCCAGGT TTTTTGCCACTTTAGCTGCG 50.0 45.0 57.9 57.9 638-644 bp 1.0 82.9
atpH-atpI_p2 GTCCAATAGAAGCAAGCCCA TTTTTGCCACTTTAGCTGCG 50.0 45.0 57.9 57.9 620-626 bp 1.0 82.9
atpH-atpI_p3 GCTGTACCTTGACCAACTCC TTTTTGCCACTTTAGCTGCG 55.0 45.0 58.2 57.9 642-648 bp 1.0 82.3
atpH-atpI_p4 TACCTTGACCAACTCCAGGT GCGGGATTCAAGCTCTCATT 50.0 50.0 57.9 58.3 656-662 bp 1.0 81.2
atpH-atpI_p5 GTCCAATAGAAGCAAGCCCA GCGGGATTCAAGCTCTCATT 50.0 50.0 57.9 58.3 638-644 bp 1.0 81.2