Hotspot

trnS-atpA

LSC · 7806-9779 · 1974 bp

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Pi 0.0303
MarkerSeek score 65.50
Primer available yes
Label trnS-atpA

Feature Metadata

label_name
trnS-atpA
feature_type
igs
region
LSC
length_bp
1974
variable_sites
85
pi
0.0303
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-atpA_p1 CCAACGCTTTAGTCCACTCA GGGTCGTGAAGCTTATCCAG 50.0 55.0 57.8 58.1 2636-2684 bp 1.0 83.2
trnS-atpA_p2 CCAACGCTTTAGTCCACTCA GGTCGTGAAGCTTATCCAGG 50.0 55.0 57.8 58.1 2635-2683 bp 1.0 83.2
trnS-atpA_p3 AACGCTTTAGTCCACTCAGC GGGTCGTGAAGCTTATCCAG 50.0 55.0 58.2 58.1 2634-2682 bp 1.0 82.9
trnS-atpA_p4 AACGCTTTAGTCCACTCAGC GGTCGTGAAGCTTATCCAGG 50.0 55.0 58.2 58.1 2633-2681 bp 1.0 82.9
trnS-atpA_p5 CCAACGCTTTAGTCCACTCA GTTTCGAGAGTAGGATCCGC 50.0 55.0 57.8 57.9 2440 bp 0.333333 51.0