Hotspot

rps16-trnQ

LSC · 6112-6542 · 431 bp

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Pi 0.0444
MarkerSeek score 75.10
Primer available yes
Label rps16-trnQ

Feature Metadata

label_name
rps16-trnQ
feature_type
igs
region
LSC
length_bp
431
variable_sites
27
pi
0.0444
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 493-504 bp 1.0 84.4
rps16-trnQ_p2 CCTTCAATTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 503-514 bp 1.0 83.5
rps16-trnQ_p3 TCAATTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.3 58.1 500-511 bp 1.0 80.1
rps16-trnQ_p4 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 497-508 bp 1.0 79.6
rps16-trnQ_p5 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 523-534 bp 1.0 79.2