Hotspot

trnH-psbA

LSC · 76-349 · 274 bp

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Pi 0.0745
MarkerSeek score 81.00
Primer available yes
Label trnH-psbA

Feature Metadata

label_name
trnH-psbA
feature_type
igs
region
LSC
length_bp
274
variable_sites
30
pi
0.0745
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-psbA_p1 CTGCCTTAATCCACTTGGCT GCTGTTGAGGCTCCATCTAC 50.0 55.0 57.9 58.1 332-358 bp 1.0 86.8
trnH-psbA_p2 ACTGCCTTAATCCACTTGGC GCTGTTGAGGCTCCATCTAC 50.0 55.0 58.2 58.1 333-359 bp 1.0 86.6
trnH-psbA_p3 CTGCCTTAATCCACTTGGCT CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 347-373 bp 1.0 86.4
trnH-psbA_p4 CTGCCTTAATCCACTTGGCT TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 350-376 bp 1.0 86.3
trnH-psbA_p5 ACTGCCTTAATCCACTTGGC CCTCTAGACCTAGCTGCTGT 50.0 55.0 58.2 57.9 348-374 bp 1.0 86.2