Hotspot

ndhA

SSC · 124251-126548 · 2298 bp

Back to Menispermum

Pi 0.0224

MarkerSeek score 70.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2298
variable_sites: 51
pi: 0.0224
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAATTACCATAGGAACCA	TGGGAGAAGTTGATCGGTGA	42.9	50.0	56.3	58.4	2415-2424 bp	1.0	57.9
ndhA_p2	AACCCAATTACCATAGGAACCA	TGGGAGAAGTTGATCGGTGA	40.9	50.0	57.0	58.4	2416-2425 bp	1.0	55.8
ndhA_p3	ACCCAATTACCATAGGAACCA	ATGGGAGAAGTTGATCGGTG	42.9	50.0	56.3	57.3	2416-2425 bp	1.0	55.2
ndhA_p4	ACCCAATTACCATAGGAACCA	GGGAGAAGTTGATCGGTGAA	42.9	50.0	56.3	57.2	2414-2423 bp	1.0	54.7
ndhA_p5	GAACCCAATTACCATAGGAACCA	TGGGAGAAGTTGATCGGTGA	43.5	50.0	58.1	58.4	2417-2426 bp	1.0	54.4