Hotspot

ycf1-trnN-GUU

SSC · 134143-134471 · 329 bp

Back to Menispermum

Pi 0.0304

MarkerSeek score 70.60

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: SSC
length_bp: 329
variable_sites: 10
pi: 0.0304
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	GTAGAACCCAACCACAACGA	AGGATTCGAACCTACGACCA	50.0	50.0	57.8	58.2	469-509 bp	1.0	78.4
ycf1-trnN-GUU_p2	TAGAACCCAACCACAACGAC	AGGATTCGAACCTACGACCA	50.0	50.0	57.8	58.2	468-508 bp	1.0	78.4
ycf1-trnN-GUU_p3	AGTAGAACCCAACCACAACG	AGGATTCGAACCTACGACCA	50.0	50.0	57.8	58.2	470-510 bp	1.0	78.4
ycf1-trnN-GUU_p4	GTAGAACCCAACCACAACGA	AAAGCGAGCCCTTTCATTCT	50.0	45.0	57.8	57.8	527-567 bp	1.0	77.9
ycf1-trnN-GUU_p5	TAGAACCCAACCACAACGAC	AAAGCGAGCCCTTTCATTCT	50.0	45.0	57.8	57.8	526-566 bp	1.0	77.9