Hotspot

ycf1-ndhF

SSC · 113485-113578 · 94 bp

Back to Menispermum

Pi 0.391

MarkerSeek score 74.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 94
variable_sites: 36
pi: 0.391
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	TCGTAGTAGCTCCGTTCCAT	TGATCAAGAAGGAGGGTGGT	50.0	50.0	58.0	58.0	877-924 bp	1.0	84.5
ycf1-ndhF_p2	GAGCTACTGAGGAACAACGG	TGATCAAGAAGGAGGGTGGT	55.0	50.0	58.0	58.0	984-1031 bp	1.0	84.3
ycf1-ndhF_p3	GCTTCCTTCGTAACTCTCGG	TGATCAAGAAGGAGGGTGGT	55.0	50.0	58.1	58.0	904-951 bp	1.0	84.0
ycf1-ndhF_p4	GAGCTACTGAGGAACAACGG	AGAAACCTCCCTGTATCCCC	55.0	55.0	58.0	58.1	1086-1133 bp	1.0	83.4
ycf1-ndhF_p5	TCAAAGCGAGCCCTTTCATT	TGATCAAGAAGGAGGGTGGT	45.0	50.0	58.1	58.0	1093-1140 bp	1.0	83.1